FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12896283
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT297280.23051603318568614No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT271960.2108824690028902No Hit
CACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCT246600.19121788813102192No Hit
CACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTT179630.13928819645164425No Hit
CTTCTGTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTAT170260.13202253703644687No Hit
CGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCTCT142780.11071407164374417No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT12950.034.846841
CGACCTA15750.027.155564
GACCGTT18850.023.4411222
GCATTCG22650.022.6188093
ACCGTTG19450.022.2320563
CCGTTGA20150.021.9255624
CGCGGTC15500.020.9256671
CCGTATG19800.020.8888934
GTCGTAA14900.020.8176655
CGTATGG20900.019.7884035
GTCAACC89600.019.6692356
CTTAGCG13500.019.4941068
GCGATTT20100.019.40570494
TCGTAAG16750.019.3596956
GCGGGTC38150.019.2217082
CATTCGG27500.019.141824
GCCTATC55500.018.7985466
CGTTGAC23800.018.3645535
ACGTGCG16550.018.1731858
GCGGTCG17300.017.933282
Produced by FastQC (version 0.11.5)