FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827746

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827746
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12923677
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT298370.2308708272421231No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT274310.21225383457045546No Hit
CACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCT247300.1913542097964844No Hit
CACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTT181790.14066430165346905No Hit
CTTCTGTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTAT174790.135247886495461No Hit
CGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCTCT143240.11083532960472474No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT14150.032.894421
CGACCTA15250.029.2806914
ACCGTTG18500.025.4100933
GACCGTT18500.025.1591072
CTTAGCG14050.023.413898
CCGTTGA20150.023.3266564
CGTTGAC21500.022.7355565
GCGATTT20200.021.8676294
CCGTATG21100.021.8308774
ACGTGCG18500.021.0842558
GTCAACC94200.020.2558546
GCATTCG21200.020.178253
CGTATGG22600.020.1731645
TCAGACG28400.019.5341912
CTACCGT26700.018.8415241
ACCGTAT25950.018.4773963
GTCGTAA13050.018.368365
TCAACCC103250.018.2519637
CTTGACG18200.018.0829871
TACGCAC25000.018.046029
Produced by FastQC (version 0.11.5)