FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12728600
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT251590.1976572443159499No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT238250.18717690869380765No Hit
CACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCT203950.1602297189007432No Hit
CACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTT160030.125724745847933No Hit
CTTCTGTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTAT132200.10386059739484312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT12100.031.0827711
CGACCTA13350.025.3511714
ACGTGCG20450.024.1283978
GACCGTT16650.022.869152
ACCGTTG18550.021.7937553
GCGATTT23350.021.73369294
CCGTTGA17500.021.4881364
TAACACG23100.020.9590724
GTCTAAC24400.020.0381951
GTCAACC79800.020.02376
GCCTATC53450.019.255946
CTTAGCG13000.019.1586538
CGTTGAC21100.018.7113935
GCGAACG6900.018.394683
AACACGT28000.018.296915
CGTATGG20600.018.0247445
CCGTATG20350.018.0167734
TCAGACG29500.017.3693562
TCAACCC93200.017.1439467
TCACCCG33700.016.3205013
Produced by FastQC (version 0.11.5)