FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12728826
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACG198450.1559059727896351No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA19000.025.9671294
CCCGTAT23000.024.795231
CGTATCG31000.022.1298983
CCGTATC37050.019.150512
TGTCGAG15050.018.4205995
ATCGCGT6450.018.22727694
TATCGAA29450.017.5507475
TAAGCCG83450.016.92571866-67
ACGTACG8200.016.33172452-53
TCGACGA86550.016.09703444-45
GACGAAA89000.015.60111846-47
CGTACGA9200.015.57890454-55
GTCCGGT12650.015.27581
CGAAACA91900.015.15993748-49
CTTAACG24100.014.8178373
GTCGAGG21450.014.6769486
CCCAAAT208250.014.66562751
GTGTCGA19650.014.5866484
CCGAGCT101650.014.22037770-71
CCCGTCT32850.014.2039941
Produced by FastQC (version 0.11.5)