FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827752

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827752
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12728826
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT252960.19873003213336407No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT238470.1873464214217399No Hit
CACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCT203690.16002261324021555No Hit
CACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTT157850.1240098654817027No Hit
CTTCTGTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTAT132360.10398445229748603No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA14100.026.333844
ACGTGCG18750.026.3166798
GTCTAAC23400.024.3075161
CGACCGT13450.024.1155871
TAACACG22050.022.1682264
GCCTATC54500.020.0058386
TAGGTCG11100.019.478393
GCGATTT22600.019.33819294
AACACGT26200.019.1938195
TCAGACG27250.018.1130732
GCGAACG8350.018.0128173
GTTAGTT63050.017.3716831
GTCAACC78850.017.2846896
CCGTTGA19150.017.1804874
ACCGTTG19450.016.9159583
CCGTATG20050.016.878134
GACCGTT19250.016.8495252
ACACGTG30700.016.8391286
ACACGCC159300.016.6395765
CCCCACA161100.016.6322271
Produced by FastQC (version 0.11.5)