FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12770289
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT255020.19969790816793576No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT237760.1861821607952647No Hit
CACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCT206370.16160166774612542No Hit
CACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTT158580.12417886549004489No Hit
CTTCTGTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTAT133180.10428894757197743No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA15450.025.554564
CGACCGT12800.025.343841
ACGTGCG18250.025.2360448
GCGATTT24500.021.86601494
GCCTATC53450.021.3665966
CCGTATG20500.020.6350864
GTCTAAC24350.020.2732621
TAACACG23000.019.8226414
TAGGTCG10000.019.7432173
CGTATGG22300.019.179575
ACCGTTG17650.018.6432653
GACCGTT17400.018.6432322
GTCAACC77450.018.5078186
GTTAGTT64000.018.36511
CTACCGT24800.018.1992231
CTTGACG18300.017.983751
CACGCCG28200.017.8341454
CCGTTGA19250.017.8241864
TCACCCG34900.017.7794023
AACACGT26700.017.7792075
Produced by FastQC (version 0.11.5)