FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827760

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827760
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13546420
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACG183450.13542323359234396No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT25250.027.9977651
GTATCGA19250.026.3618894
CGTATCG33350.020.5702933
ATCGCGT6950.018.9468494
CCGTATC39600.018.3916192
TAAGCCG69200.017.15103366-67
TCGACGA69950.017.02839744-45
GACGAAA73600.016.24775946-47
TATCGAA32900.015.9957875
CCCAAAT192300.015.3912491
CGAAACA79250.015.1486948-49
CCCGTCT36800.015.11221
CTTAACG34750.014.73856453
ACGTACG7250.014.25884152-53
CTCCGAT33750.014.1039611
AACGGTT37150.014.0393716
TAACGGT38550.013.8951725
CCGAGCT87300.013.892838570-71
TGTCGAG15900.013.8894245
ACCCGTT25500.013.8198892
Produced by FastQC (version 0.11.5)