FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827761

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827761
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13546420
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT231180.17065763500614922No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT214780.15855111535003344No Hit
CACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCT184350.1360876157685942No Hit
CACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTT141450.10441873203399865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA20100.029.6968364
CGACCGT14050.028.4388791
ACCGTTG18450.022.6729163
GACCGTT19800.020.8923532
CCGTTGA19900.020.7841434
CGTTGAC21200.020.6168525
TACGCAC28400.020.35339
GCGATTT24000.020.16844494
CTTAGCG14250.020.1171368
GCCTATC54350.019.9744536
TGACGAC31300.019.6741891
CTTGACG19500.019.285251
GCATTCG20300.019.2174533
TAGGTCG11750.019.20073
TCAGACG30050.019.0846942
GACGACC35250.018.8031182
GTCGTAA14700.017.9038315
ACGACCT36700.017.6736723
CGCGGTC18950.016.620171
ACACGCC148600.016.5408635
Produced by FastQC (version 0.11.5)