FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13577675
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACG183120.13486845133647696No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT24500.024.6255971
GTATCGA20200.023.0277964
CGTATCG34250.020.4406263
TAAGCCG67950.017.6425666-67
CCGTATC41950.017.4727442
TCGACGA71200.016.9602144-45
GACGAAA73250.016.26104446-47
ATCGCGT7950.015.97392294
CGCGTAA5450.015.56751
CGAAACA79000.015.19644248-49
TATCGAA32250.014.8606885
CCCGTCT35850.014.0681671
CCGAGCT86950.014.00459870-71
CTCGTTT44950.013.9464861
CCCAAAT200150.013.8001851
TGTCGAG16500.013.6686565
AGTTTAC122300.013.49881494
CCGGTAT84200.013.40114972-73
CCCGAAC20450.013.3683421
CTCGTAT16600.013.3454331
Produced by FastQC (version 0.11.5)