FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827767

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827767
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13403582
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCT616700.46010088944880556No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT347720.25942319000995406No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT319330.23824228478626086No Hit
CGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCTCT269490.20105819474227116No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT261650.1952090120387222No Hit
CACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTT259010.19323938929160875No Hit
CTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCTCTTTA237610.1772735079324318No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG205680.15345151766147289No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCC196000.14622956758872369No Hit
CTCATTCTTGGCAGGATGGCTTCTCATCGTCTGCTCCTCCTCTGCCTTGC189090.14107422926199878No Hit
CAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTT187320.1397536867383659No Hit
CCAAGATGAGTGCCCTGGGAGCTGTCATTGCCCTCCTGCTCTGGGGACAG186360.13903746028487013No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCC171850.12821199586797022No Hit
CCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTACT171380.12786134333344623No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC167060.1246383242927152No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC157830.11775210537004212No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA156860.11702841822432242No Hit
CTTTATTTCCCTTCTTTTTCTCTTTAGCTCGGCTTATTCCAGGGGTGTGT138000.10295755269001973No Hit
CTTCTGTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTAT134700.10049552425612795No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT11800.058.5090681
ACCCGGC57250.044.2183765
ACCGTTG17050.039.6665463
GCGATTT59800.038.99444294
CCAGTAG56900.037.886975
TCACCCG67100.037.867013
GACCGTT19050.035.5020752
CACCCGG74200.033.990434
CGGCCCG81300.031.3109748
TAGCATA73800.030.1656789
CCTAGAG67850.028.6575051
GTAGCAT79050.028.399718
CGGACAC57200.027.79536412-13
TAACCCC40750.027.2004159
CTCGATT57650.026.90756848-49
CCGTATG36050.026.5773164
GTCTAAC12250.026.4545781
ATTCCGT57450.026.43026552-53
GTTAGTT64750.026.3302331
CGGACAG61350.025.57603318-19
Produced by FastQC (version 0.11.5)