FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13346017
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA237300.17780585773268534No Hit
CAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACGAA233230.17475625873996714No Hit
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACG207380.15538718405648666No Hit
CTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTTGC201550.15101883955340384No Hit
CTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAA197580.14804416928286543No Hit
CTTACTTTCAACAAAATCAGCAGCTAATGAAGGCAAGTCAGCAGGCATCT188080.14092594067578362No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT179940.13482674269034725No Hit
CTTGATTTGTCTCTCCTTCTCAGAAAGTGTGCATATATCTGCATGGAAGG173590.13006876883192942No Hit
CTTCAAATGGACACTGCTGAAGATACTGAGCAAAGGCAATCAACACCAAG163630.1226058681028205No Hit
CTCTTATTCTCATGGTAGGCTGAGATGCTTTTAAATGTGATGTTATAAGC162380.12166925907557287No Hit
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACAAGCTCAACAAGTGCAGTTT145480.10900630502718527No Hit
CTCATGGTAGGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCA143870.10779995260009034No Hit
CCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACA141330.10589676305672321No Hit
CTTATCGTCAGCCTTGCAGCACTTCTCTACAAAAGCTGCGAAATCATCCA140040.10493018254060368No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT138380.10368636575241885No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA26450.034.1170354
CCCGTAT28100.033.8055651
TGTCGAG10650.027.3613685
TATCGAA40150.023.6462125
CTTACTT90300.023.3310031
TCGACCG17250.022.88461530-31
CGCGTAA27600.022.6613481
ACCCGTT39250.022.631822
GTTCGAC17850.022.24702528-29
CGTATCG52850.021.9658343
CCGTATC54550.021.109052
GCGTAAC30600.021.0424612
CGCTACT4000.019.9862861
ACGACTT17350.019.786391
GTCGAGG15000.019.7382036
CCGTTTA56800.019.1143444
CGGCATG52900.018.6577553
CCCGTTT55000.018.6293
CGATCCG20950.018.39395582-83
CGTAACT36050.018.2523383
Produced by FastQC (version 0.11.5)