FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13346017
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCT617540.4627148309491888No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT344720.2582942910982355No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT322100.24134541414116287No Hit
CGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCTCT267750.200621653636437No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT262940.19701758209958822No Hit
CACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTT260210.19497202798407945No Hit
CTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCTCTTTA237320.1778208434771213No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG202320.15159579071418836No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCC194620.14582627910634313No Hit
CTCATTCTTGGCAGGATGGCTTCTCATCGTCTGCTCCTCCTCTGCCTTGC190900.14303893064125425No Hit
CAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTT186460.13971209537647075No Hit
CCAAGATGAGTGCCCTGGGAGCTGTCATTGCCCTCCTGCTCTGGGGACAG186000.13936742325444362No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCC172980.12961170362663257No Hit
CCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTACT168670.12638227570068283No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC167820.12574538156215448No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA157170.11776547265000487No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC156690.11740581478354178No Hit
CTTTATTTCCCTTCTTTTTCTCTTTAGCTCGGCTTATTCCAGGGGTGTGT135220.10131861813153693No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT11550.053.686011
ACCCGGC60500.047.2080155
GCGATTT63500.042.03991794
TCACCCG70200.040.8860243
ACCGTTG16100.039.9728853
CCAGTAG59000.036.783885
CACCCGG79300.036.253154
GACCGTT17950.034.806182
CGGCCCG84300.033.990198
TAGCATA71450.031.3596129
CCTAGAG66350.029.8064561
CGGACAC59650.029.49320412-13
CTCGATT58900.029.21375548-49
CGGACAG61250.028.38252618-19
ATTCCGT59700.028.31109852-53
CGACCTA7850.028.1252084
GTAGCAT79100.028.0889788
CGTTCTT61500.027.13689676-77
GTCTAAC12350.027.006021
AGTACGC53100.026.5169748-49
Produced by FastQC (version 0.11.5)