FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13436209
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA240810.17922466076554777No Hit
CAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACGAA237540.17679093857501027No Hit
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACG208880.15546051717415232No Hit
CTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTTGC206540.15371895450569428No Hit
CTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAA201880.15025071431979065No Hit
CTTACTTTCAACAAAATCAGCAGCTAATGAAGGCAAGTCAGCAGGCATCT190310.14163965445908142No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT183250.13638519615168238No Hit
CTTGATTTGTCTCTCCTTCTCAGAAAGTGTGCATATATCTGCATGGAAGG175220.1304088080201789No Hit
CTTCAAATGGACACTGCTGAAGATACTGAGCAAAGGCAATCAACACCAAG165950.1235095405259028No Hit
CTCTTATTCTCATGGTAGGCTGAGATGCTTTTAAATGTGATGTTATAAGC164920.12274295524876101No Hit
CTCATGGTAGGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCA147000.10940585994159513No Hit
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACAAGCTCAACAAGTGCAGTTT146130.10875835587255303No Hit
CTTATCGTCAGCCTTGCAGCACTTCTCTACAAAAGCTGCGAAATCATCCA142820.1062948633799906No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT140870.10484356115627556No Hit
CCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACA139620.10391323921799668No Hit
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACGAGCTCAACAAGTGCAGTTT135330.10072037432582359No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA26200.030.468024
TGTCGAG10650.028.6589535
CCCGTAT30100.027.938911
CGCGTAA29250.024.7353321
GTTACGA6450.024.0243034
CTTACTT90950.023.761731
GTCGAGG12850.023.7522756
GCGTAAC33050.022.3059922
TATCGAA40550.022.0018565
CGCGTTT3500.021.477061
CGTATCG50500.021.107223
ACCCGTT39450.021.0678232
TCGACCG19150.020.84367230-31
CCGTATC52250.020.6697352
GTTCGAC19000.020.63760828-29
ACGACTT18800.020.2418441
TTACGAC9400.019.981535
CGTAACT36950.019.8247053
CACGGAA38850.018.97595494
CGGCATG49950.018.4254113
Produced by FastQC (version 0.11.5)