FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13436209
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCT618410.46025631188082894No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT350250.2606762071057394No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT324820.24174973759339408No Hit
CGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCTCT266900.19864234026130437No Hit
CACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTT260710.1940353860229474No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT260020.19352184831301747No Hit
CTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCTCTTTA239180.17801152095803213No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG204750.15238673349007892No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCC198960.1480774822719712No Hit
CTCATTCTTGGCAGGATGGCTTCTCATCGTCTGCTCCTCCTCTGCCTTGC189730.14120798507972004No Hit
CAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTT188490.14028510571694738No Hit
CCAAGATGAGTGCCCTGGGAGCTGTCATTGCCCTCCTGCTCTGGGGACAG184630.13741227157154223No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCC174920.13018553075499198No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC170490.12688846980573168No Hit
CCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTACT169590.12621863801017089No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA158890.11825508221850374No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC158730.11813600101040404No Hit
CTTTATTTCCCTTCTTTTTCTCTTTAGCTCGGCTTATTCCAGGGGTGTGT139950.1041588442097023No Hit
CTTCTGTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTAT136340.10147207445195293No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT11950.058.582571
ACCGTTG15500.045.771173
ACCCGGC60500.045.6627735
GCGATTT66800.039.26450794
TCACCCG73800.037.6251223
GACCGTT18850.036.6397742
CCAGTAG55750.035.732335
CACCCGG80250.034.8347174
CGGCCCG84650.032.7464188
CGGACAC59050.029.20171212-13
CGACCTA8700.029.1618354
CTCGATT59200.028.74088748-49
GTCTAAC12750.028.3746531
ATTCCGT59150.028.28904252-53
TAGCATA72850.027.6027899
GTAGCAT74550.027.0993928
CCTAGAG68450.026.9754771
CGGACAG64100.026.72094218-19
AGTACGC47900.026.19926348-49
CGTTCTT64800.025.57256176-77
Produced by FastQC (version 0.11.5)