FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12179609
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAGATGAGTGCCCTGGGAGCTGTCATTGCCCTCCTGCTCTGGGGACAG132920.10913322422747725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT11550.040.2587741
CATTCGG28850.036.7934074
GCATTCG35400.030.2509443
ACCGTTG16350.029.3014973
ACCCGGC44150.027.9793575
GCGATTT42050.027.6153494
TCACCCG50800.024.4088653
GACCGTT18500.024.372882
CTACCGT27450.024.1259331
CCGTATG24900.023.955864
CCGTTGA19350.023.7876994
ATGACGG59750.022.89678494
CGTATGG27400.022.2846345
CGGCCCG55150.022.058048
TCGGGCC53300.021.0612147
TAACCCC36350.020.5450529
CTCGATT37100.020.52473648-49
ATTCGGG51650.020.4609095
CACCCGG62100.020.3454844
ATTCCGT38250.019.8477652-53
Produced by FastQC (version 0.11.5)