FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827787

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827787
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12067470
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT27050.028.1638091
GTATCGA22900.027.7077124
CGTATCG28900.024.394723
CCGTATC33600.022.940622
TATCGAA29400.022.3811875
CGTACGC8650.021.7343335
CCCGTCG18550.021.548581
TTCGCGC5750.020.4316947
TCGACCG13800.019.41059330-31
CCGTACG9500.019.2949394
GTTCGAC15100.017.73948128-29
CGGCATG43050.017.5774213
CTCCGTT27550.016.5574631
ATCGAAG46150.016.1910746
GTTCCGA29300.015.7184126
CGATCCG17300.015.48314682-83
TACGCCA12600.015.2913417
ACGACTT17700.014.8784751
ATTCGCA34800.014.85401690-91
GAGCGAC35850.014.62061154-55
Produced by FastQC (version 0.11.5)