FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827790

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827790
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12144895
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT25450.026.7687931
GTATCGA19150.026.727794
CGTATCG27600.022.2878173
TATCGAA28150.020.6845865
CGCGTAT2057.5973367E-720.6270561
CCGTATC32550.020.1963752
TCGACCG14200.019.8413830-31
CGTACGC9950.019.3492585
GTTCGAC14850.018.3402828-29
CCGTACG10850.017.7443244
CCCGTCG18500.017.5237061
CGATCCG16500.016.50560682-83
CGGCATG42400.016.390843
GTTCCGA30850.016.1342536
ATCGAAG43700.016.1178826
GTACGCC12900.016.0162626
CTCGTTT36950.014.7500031
GCAAGCG38700.014.681149594
TTCCGAT34550.014.6780557
CGCGTAG4251.9865183E-814.371531
Produced by FastQC (version 0.11.5)