FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827793

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827793
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9734594
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT222040.22809374484441775No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT215430.2213035284265579No Hit
CACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCT210440.21617748002638837No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT182800.187783897304808No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT179100.18398301973354No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA160410.16478345167759437No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC143920.1478438648802405No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAACCAAACCATTTAC138470.1422452749441836No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA135620.13931757195009878No Hit
CTCATTCTTGGCAGGATGGCTTCTCATCGTCTGCTCCTCCTCTGCCTTGC131110.13468461036998564No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCC122480.12581932025105516No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG118380.12160753699640683No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG117170.12036454730418135No Hit
CACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTT115400.11854628965522342No Hit
CTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCTCTTTA113110.11619385461787107No Hit
CCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTACT110680.11369760259133559No Hit
CTTCTGTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTAT109440.1124237949728566No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCC108300.11125271377522268No Hit
CAAGGATTCAGAGTGCCCCTCCGGCCTCGCCATGAGGCTCTTCCTGTCGC106520.10942418348418023No Hit
CGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCTCT106050.10894136930620835No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG105630.10850991833865901No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT103220.10603421159629256No Hit
CCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTT98760.10145261322660196No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT10350.063.6366651
ACCGTTG13450.050.026893
ACCCGGC45800.043.8674355
TCACCCG46150.043.739653
GACCGTT16550.039.7971532
CACCCGG51250.039.3874244
CGCAAAA34050.033.120191
CGGCCCG61700.032.565548
CCAGTAG38050.031.257985
TAACCCC34550.030.999589
GTCTAAC9700.029.7982671
CTCGATT39550.029.78295348-49
CGGACAC41000.029.13176312-13
ATTCCGT40650.028.51919252-53
CCGTTGA23700.028.191144
TCAGACG25050.028.184722
CCCGGCC74650.027.1046876
GTAGCAT45650.027.094268
TAGCATA46300.026.8173439
GTTAGTT59150.026.6761071
Produced by FastQC (version 0.11.5)