FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827800

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827800
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10450102
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGA194230.18586421453111174No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG148170.1417880897239089No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCGTA50650.038.9176068
TGTCGAG15350.030.0151715
CGTACGC17500.029.3131945
TTACGAC9300.029.1118225
GTATCGA20750.029.071214
GTTACGA9250.028.7556884
CCCGTAT24200.028.560671
CAGGCGT70050.028.3429877
CCGTACG18150.028.2634124
CCCGTCG24850.025.8954321
GTCGAGG17850.025.8113656
TGTTACG10150.025.737963
CTCAGAC96650.025.6951791
GGCGTAG78500.025.6550989
CGGCATG61600.025.291273
ACAGGCG80950.024.7612766
TGTGTCG19550.024.2957733
CGCAAGT44850.024.19921136-37
ACGTACG9350.024.1301252-53
AAGTGCG41350.024.12244238-39
Produced by FastQC (version 0.11.5)