FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10450102
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT582320.5572385800636204No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG311450.2980353684586045No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAACCAAACCATTTAC173560.16608450329001573No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC162400.15540518169104953No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC161290.15434299110190502No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA157830.15103201863484203No Hit
CTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAATGTG152020.14547226428986051No Hit
CGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGACGGAACAGATCCGGGG149860.14340529881909286No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG110850.053.3972743
ACCCGGC110400.053.357885
CACCCGG117650.050.1503454
CGACCGT12150.048.3657341
CGGCCCG124300.047.281678
CCAGTAG70750.044.0753675
GCCAGTA77950.040.308254
CTCACCC150100.039.5921632
ACCGTTG15250.039.4662863
CAGTAGC82300.038.293646
CCGGCCC159400.037.2832767
GTAGCAT86850.036.508888
TAGCATA88500.036.0443239
CCCGGCC166950.035.7391436
AGTAGCA89150.035.6170777
CGGACAC114100.032.98666412-13
CTCGATT113500.032.8512848-49
GTGCGTC41850.032.731629
GACCGTT18450.032.621182
ATTCCGT115500.031.90246452-53
Produced by FastQC (version 0.11.5)