FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827802

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827802
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10439029
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGA198900.19053496259086933No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG151620.14524339380607143No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCGTA51400.038.9041638
CCCGTCG24250.028.700091
GTATCGA23750.028.3989224
CCCGTAT25850.028.2145441
CAGGCGT71450.028.1864747
GGCGTAG77300.025.99199
CGTACGC16200.024.9218965
ACAGGCG81550.024.7538156
GTTACGA8700.024.5680354
TTACGAC8900.024.0159425
CTCAGAC99900.023.8586921
TGTCGAG13900.023.5782415
AAGTGCG42150.023.55189538-39
CCGTACG17200.023.4729484
CGCAAGT45550.023.25378436-37
CGGCATG64400.022.7902853
GACAGGC90500.022.7256575
GCGTAGC50200.022.42444810-11
CGTTCGA47900.022.26168344-45
CGCGTAT1302.873587E-422.0013871
Produced by FastQC (version 0.11.5)