FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827808

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827808
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8379676
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGAAA12700.029.5462442
AGGCGAA30200.028.2438561
GCGAAAA7900.026.45483
CGTACGC16950.025.2203875
ACGCGAT1151.2552338E-424.8617291
CCGTACG17700.024.1517244
TGTCGAG10500.023.975385
GTATCGA20750.023.8064234
GTACGCC19300.022.3956156
CCCGTAT23850.021.97771
TTCGCGC10600.021.5087057
TCCGTAC20650.020.9314963
TCGACCG12100.020.41253330-31
TATCGAA28500.020.3326455
TACGCCA21650.020.1840747
GTTCGAC12650.019.33729228-29
GTCGAGG13650.019.1385466
CGCGTAA15550.018.9993691
ACCCGTT33450.018.7437612
ACGTACG6600.018.71148752-53
Produced by FastQC (version 0.11.5)