FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827812

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827812
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10550027
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA29300.028.0450784
CCCGTAT32600.027.484541
TGTCGAG11900.024.3478745
TATCGAA38500.023.6874685
CGTACGC20150.021.6865735
CGTATCG45550.021.3768483
CCGTATC46850.021.0878312
CCGTACG20600.020.9822654
ACGTACG6650.020.3564252-53
GTCGAGG15100.019.8171776
CGCGTAA7500.019.6992021
ACCCGTT48050.018.9795552
CGTACGA8000.018.70246154-55
GTACGCC24250.018.019986
CACCCGT52900.018.0186611
TCCGTAC25400.017.9521183
TACGCCA25250.017.4944297
ATCGAAG63400.016.9315746
TCGAGGG17900.016.1865757
TTCGCGC12250.015.5096527
Produced by FastQC (version 0.11.5)