FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10579915
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAACCAAACCATTTAC201680.19062535001462677No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC200830.18982194091351395No Hit
CGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGACGGAACAGATCCGGGG164370.15536041641166304No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA116170.10980239444267748No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA113100.10690066980689353No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT108020.10209911894377224No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT11750.056.8652761
ACCGTTG16300.040.9918373
GACCGTT17900.037.59252
CCGTTGA19550.034.419734
GCGGGTC55050.030.9031662
CTTAGCG14000.029.44928
GCATTCG22950.028.0816153
CATTCGG22850.027.5823524
TAACCCC37950.027.2234679
CGCAAAA40950.026.7315371
GCCTATC69350.026.5130276
CCCAAAC94900.026.2654821
AACCCAC84450.026.0368425
ACTACCG29800.024.1714927
CGTTGAC30050.023.4969645
ACATGCC81250.023.3293422
ACCCGGC34450.022.8343585
GTTAGTT56600.022.6891771
CGCGGGT77700.022.6875611
CTACCGT31350.022.5232498
Produced by FastQC (version 0.11.5)