FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827817

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827817
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10874102
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGACGGAACAGATCCGGGG171850.15803603828619597No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC131040.12050650251395471No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT10250.043.4492531
GCGGGTC59550.031.347062
GCATTCG25600.029.6116333
CATTCGG27050.028.5501674
ACCGTTG15750.027.6751253
CCGTTGA16750.026.588964
GACCGTT16850.025.868442
GCCTATC54600.023.6034016
CGCGGGT83150.023.2475011
ACGACGT10650.022.2446067
TAGGTCG8350.021.5615233
CTTAGCG14400.021.0590978
GTCTGTC89900.020.9759546
GGTCTGT90950.020.8897885
TAACCCC33200.020.8383359
ACCCGGC36400.020.3055885
GTTAGTT54450.020.1868461
CTACCGT21300.020.0209738
CCGTATG18500.019.9761224
ACCTAGC32800.019.6455146
Produced by FastQC (version 0.11.5)