FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10942806
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGACGGAACAGATCCGGGG172880.15798507256731043No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC132580.12115722420739251No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT11750.044.362461
GCATTCG26100.035.7674943
CATTCGG26900.033.646964
ACCGTTG15800.032.391383
GCGGGTC62800.032.2202572
GACCGTT16450.030.8232652
CCGTTGA17750.029.9009114
GCCTATC57850.026.6228586
CGCGGGT86800.024.2941131
TAACCCC32550.022.7145379
CACATGC71350.022.7139641
GTCTGTC91600.022.245786
GGTCTGT93250.022.1057455
ACATGCC72900.021.9710062
TCGGGCC43800.021.0976077
CTTAGCG15100.021.0285958
GTTAGTT56800.020.5233171
CCCAAAC74950.019.9791151
ATTCGGG44850.019.969365
CGCAAAA40950.019.788051
Produced by FastQC (version 0.11.5)