FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10965912
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA21550.027.5512524
CCCGTAT25400.026.2660711
ACGTACG7100.020.73872252-53
TGTCGAG14350.020.521945
CGTATCG36250.019.9165553
TATCGAA34850.019.6262955
CGTACGA7900.018.9392254-55
ACCCGTT31050.018.8157772
CCGTATC38350.018.7020932
GTCGAGG18950.018.5481726
CGCGTAA7500.017.7908841
CGCGATT2152.3316052E-517.731781
CGTACGC19750.017.3158595
CACCCGT37100.017.0835651
CCGTACG20150.016.972124
CTCGTTT45250.016.95541
TTCGCGC12800.016.6986437
TCGAGGG18700.016.2561247
TCGACTG25600.016.1420212
CTCGACT30850.016.0649341
Produced by FastQC (version 0.11.5)