FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827821

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827821
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10965912
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGACGGAACAGATCCGGGG172490.15729653858247267No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC133550.12178649618928183No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT11900.042.6105041
GCATTCG24600.033.3265883
CATTCGG26100.032.5006754
GCGGGTC62600.030.8105832
GACCGTT16150.030.8103492
ACCGTTG17900.027.7981663
CCGTTGA18450.027.740054
CTTAGCG14300.024.5242278
GCCTATC57700.024.2286976
ACCCGGC36400.023.0438865
TAACCCC33350.022.8797639
CGCGGGT86900.022.8493651
CCGTATG19450.022.6591284
TACACGC15750.021.9645422
GTTAGTT57250.021.9356371
CGCAAAA39450.021.862721
CTCGATT23550.021.37878848-49
TCACCCG39850.021.1675973
GTCTGTC90900.020.645016
CTACCGT22750.020.6221371
Produced by FastQC (version 0.11.5)