FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13094992
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA29350.030.7484024
CCCGTAT34150.027.778961
CGCGTAA17700.023.7007981
TATCGAA47300.022.2930185
CGTATCG48450.022.0579833
ACCCGTT36700.021.0959242
CCGTATC52100.021.0596582
TGTCGAG17300.020.8662575
GTCGAGG20400.019.7909136
ACGTACG8400.019.7909152-53
GCGTAAC21150.019.5382632
CGTACGC21950.019.4753385
CGTACGA9200.018.32810654-55
CCGTACG23350.018.1042354
CACCCGT46550.017.7165831
TCGAGGG22050.017.017497
ATCGAAG71350.016.9755276
AGGCGTA30300.016.9300488
GTACGCC27750.015.7471486
TCCGTAC28400.015.3867373
Produced by FastQC (version 0.11.5)