FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13094992
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGACGGAACAGATCCGGGG226220.1727530646830483No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC224530.1714624949751783No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT211850.16177940391257972No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA142750.10901113952570571No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA133560.10199318945746587No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT12350.040.6687661
ACCCGGC62650.036.4546555
GCGGGTC75400.033.0549432
CATTCGG30800.032.3069234
TCACCCG70200.032.061233
GCATTCG31050.031.5887493
TAACCCC46400.031.5600159
CACCCGG73000.031.1562654
GCCTATC81500.030.2334486
CGCAAAA52300.028.9008791
CGGCCCG79350.028.7858168
CTCGATT49000.028.49848-49
CACATGC98250.026.4765761
ACCGTTG18750.025.776423
ATTCCGT54300.025.46351852-53
CTACCGT34700.025.398351
ACATGCC99550.025.2740782
GACCGTT18950.025.2542342
CCGTATG34200.024.8001294
GTTAGTT65100.024.5284981
Produced by FastQC (version 0.11.5)