FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13134146
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA30200.026.2655264
CCCGTAT36400.025.5407141
CGTACGC22350.022.9521185
CCGTACG22950.022.3520644
TGTCGAG15200.021.2491655
CGCGTAA19050.019.771151
TATCGAA47300.019.6821045
CGTATCG49450.019.5947843
ACGTACG7900.018.9391352-53
CCGTATC53250.018.8208562
GTACGCC28300.018.4621736
CGTACGA8250.018.4235254-55
TCCGTAC28700.018.3703583
TACGCCA28500.018.3326137
ACCCGTT36650.018.1438982
GTCGAGG20450.018.1166486
GCGTAAC22150.017.369522
AGGCGTA32850.016.194198
TCGAGGG21450.015.943437
TTCCGTA38850.014.7934982
Produced by FastQC (version 0.11.5)