FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13134146
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC229620.17482674549224592No Hit
CGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGACGGAACAGATCCGGGG229010.174362307225761No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT214210.16309396895694625No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA142680.10863287190503287No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA134740.10258756069865525No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT12950.037.6897931
ACCCGGC63800.037.5086945
TCACCCG69650.034.5623
CACCCGG73150.033.0378764
TAACCCC43700.032.0997249
GCGGGTC72700.031.4824852
CGGCCCG77800.031.4298958
GCCTATC78500.029.941486
GCATTCG30100.029.9118583
CATTCGG30950.029.7027994
CTCGATT49150.029.61900348-49
CGCAAAA51100.028.46911
ATTCCGT53900.027.02106552-53
CTTAGCG16800.026.2329128
CACATGC94550.025.5602421
CCGTATG33550.025.1410714
ACATGCC95150.025.100252
ACCGTTG20100.024.5184823
CTACCGT35150.024.2662941
CGGACAC61300.024.20230512-13
Produced by FastQC (version 0.11.5)