FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11488046
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT489580.42616472810084505No Hit
CACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCT480750.41847847754091516No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT350680.3052564378659347No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT323950.28198877337364425No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA315570.2746942343371536No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC304870.2653802047798207No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG255350.2222745278004632No Hit
CACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTT221400.1927220695321032No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT219380.19096372002688708No Hit
CTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCTCTTTA218370.19008454527427904No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC210460.18319912716226938No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT207770.18085756272215484No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT201370.17528655438879684No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC193590.16851429738355853No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCC186560.1623948929173856No Hit
CCAAGATGAGTGCCCTGGGAGCTGTCATTGCCCTCCTGCTCTGGGGACAG186230.16210763780019682No Hit
CTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGA180850.1574245089199678No Hit
CGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCTCT175180.15248894372463342No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT173310.15086116472723038No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA171900.14963380195378745No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG169490.1475359691282573No Hit
CAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCCAAGGCAA153420.13354751539121623No Hit
ATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGC149890.13047475610734846No Hit
CCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTT145410.12657505027399785No Hit
CTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAG143070.12453815035211385No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC142110.12370249910211013No Hit
CTTCTGTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTAT135480.11793128265677211No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC135040.11754827583385372No Hit
CTCATTCTTGGCAGGATGGCTTCTCATCGTCTGCTCCTCCTCTGCCTTGC128920.11222099911508016No Hit
CAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTT126870.11043653550830142No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG126120.10978368296923602No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT123310.10733766212287102No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA119280.10382966781295967No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA117960.10268064734420457No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA115070.10016498889367259No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT18750.074.5329361
ACCGTTG23750.059.2414933
ACCCGGC93000.054.454955
GACCGTT26650.052.083682
CACCCGG99400.051.091564
CGGCCCG100800.050.2918978
TCACCCG103050.049.5568773
CGCAAAA44800.046.7381741
CCCGGCC115350.044.561376
CCGGCCC118550.042.8399627
TAACCCC48950.041.911639
CCGTTGA36950.038.0787544
CGGACAC88450.036.59258712-13
GTTAGTT88850.036.4158741
ACATGCC90000.035.8456042
ACTACCG51850.035.2691047
CTCACCC145050.035.1092832
CCAGTAG46000.034.8097955
CGGACAG92850.034.76439718-19
CTACCGT61100.034.269561
Produced by FastQC (version 0.11.5)