FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004827831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11518022
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT495950.43058608500660966No Hit
CACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCT478630.41554878085838No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT354540.3078132686324093No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT323350.280733966300811No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA313060.2718001406838778No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC307870.26729415866717393No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG253860.22040242673611843No Hit
CACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTT226790.1969001274698034No Hit
CTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCTCTTTA220760.1916648535660029No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT218360.18958116246001266No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC211370.18351241211381608No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT209250.1816718183035247No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT200410.1739968893964606No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC191140.16594863249957328No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCC186700.16209380395349132No Hit
CCAAGATGAGTGCCCTGGGAGCTGTCATTGCCCTCCTGCTCTGGGGACAG185240.16082622519734724No Hit
CTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGA181870.15790037560268594No Hit
CGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCTCT174130.1511804717858674No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT173790.1508852822125188No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA170180.14775106350725847No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG169840.14745587393390983No Hit
CAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCCAAGGCAA156360.13575247555526462No Hit
ATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGC148390.128832884674122No Hit
CCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTT144600.1255423891359124No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC144290.12527324570138865No Hit
CTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAG143840.12488255361901549No Hit
CTTCTGTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTAT139680.12127082236863239No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC135460.11760699884059954No Hit
CTCATTCTTGGCAGGATGGCTTCTCATCGTCTGCTCCTCCTCTGCCTTGC130100.11295342203722133No Hit
CAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTT126080.10946323943468766No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT124400.1080046556604945No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG123640.10734482014359759No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA119730.10395014005008846No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA119150.10344658136614082No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA115590.1003557728922553No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)