FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827834

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827834
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10345486
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT111730.10799879290349433No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGC16150.029.7049065
CCGTACG16700.028.4421794
GTATCGA21800.027.6711064
TGTCGAG14350.026.1489665
TATCGAA24050.025.2798335
GTACGCC20250.023.6905776
TACGCCA21000.023.0706677
CCCGTAT28750.023.0363851
GTCGAGG17150.021.3258296
ACGTACG8250.021.3023352-53
CGTCTAC19550.020.4085373
GTGTCGA17950.020.1107584
CGTACGA9350.019.05017554-55
CGTATCG40400.018.8112623
CTCCGAT45800.018.4138721
CTCGACT31900.018.3718031
CTCCCGT32000.018.0165961
TGTGTCG20800.017.5835553
TCCGTAC27550.017.413223
CCGTATC43650.017.4106522
Produced by FastQC (version 0.11.5)