FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827836

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827836
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10422728
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT113470.10886785110385687No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT105310.10103880673082902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGC15400.035.1611565
CCGTACG16300.033.219744
GTATCGA24200.030.4225314
TGTCGAG14050.028.3976215
GTACGCC19600.028.1113016
TATCGAA28050.027.2628975
TACGCCA20550.026.811757
CCCGTAT29700.026.1517011
ACGTACG8350.023.03814752-53
GTCGAGG17600.022.939576
GTGTCGA18400.022.7166294
CGTACGA10050.021.0316354-55
CGTATCG41150.020.6615163
TCCGTAC27400.019.9354613
CCGTATC44200.019.0208532
ACCCGTT33800.018.9712562
CGTCTAC21900.018.2185653
TTCCGTA31150.018.1454562
ACGCCAC30950.017.8023098
CACCCGT38750.017.3386861
Produced by FastQC (version 0.11.5)