FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10422728
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC159480.15301176429050053No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC145070.13918620921509225No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA138540.13292105483324518No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG129460.12420932408482693No Hit
CACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCT121740.11680243406524665No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA116910.11216833059444706No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT17300.052.3219221
GACCGTT21150.042.125392
ACCGTTG22200.040.9868663
CCGTTGA23200.039.0162964
CGACCTA15750.032.4970444
TAGGTCG11500.030.0830253
CGTTGAC31450.029.384395
TACACGC38000.028.6840632
CACGCCG39000.028.1918774
GCCTATC64900.026.0696136
TCGTAAG13450.025.3700456
ACGCCGC45250.024.9264875
GTTAGTT60600.024.6339681
CGCGGTC14250.024.6100961
TAACCCC40900.024.5670369
GTCGTAA13850.023.9525075
CTTAGCG17300.023.2863038
GGTCGTA14250.023.2800454
GCGGTCG14400.023.0373232
TGACGAC22750.022.7060491
Produced by FastQC (version 0.11.5)