FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10444183
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC156530.14987290054186145No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC146100.13988648034987514No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA135450.12968941658720456No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG127380.12196262742619503No Hit
CACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCT120110.11500181488585559No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA118720.11367093050744133No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT17600.050.8890341
GACCGTT20500.044.1524242
ACCGTTG22200.040.557923
CCGTTGA22600.039.6303984
CGACCTA15850.037.3728144
TAGGTCG11800.032.9311523
CGTTGAC28400.032.204625
TACACGC38350.030.2744162
CACGCCG41050.027.5905154
ACGACGT15900.027.4206837
TGACGAC23700.026.5936781
CTTAGCG18000.025.8023838
ACGCCGC45400.025.7822195
CCGTATG24750.025.273994
GTTAGTT61500.025.0428241
GCCTATC65850.023.7487956
ACCGTAT28050.022.6384663
CTACACG56650.021.8331131
GACGACC32550.021.546952
CTACCGT31250.021.533441
Produced by FastQC (version 0.11.5)