FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10222919
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA114550.11205214479347826No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT114260.1117684684775454No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT104340.10206478208425597No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA18900.034.430044
CCCGTAT24750.030.9987531
TGTCGAG15750.030.1576965
TATCGAA21600.029.9063825
CGTACGC27350.026.397635
CCGTACG28200.025.265094
GTCGAGG20300.023.164236
GTACGCC31550.023.0340756
TACGCCA32250.022.5341117
ACGTACG9650.022.149552-53
GTGTCGA24300.020.1330554
TCCGTAC36200.019.6816443
CCGGGAT29150.019.1267361
TCGGTCG20850.018.6804147
CTCCGAT39100.018.6469691
CGTATCG49200.018.439413
CCGTATC52000.018.0859172
CGGTCGC21450.017.9364478
TGTGTCG24900.017.9311123
TACGTAC12750.017.6954752-53
Produced by FastQC (version 0.11.5)