FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10222919
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA176860.1730034249513275No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC121840.11918318045951455No Hit
CTACACGCCGCCGCTTGTGCTGCAGCCATGTCTCTAGTGATCCCTGAAAA106540.1042168093085742No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT15050.044.3931541
ACCGTTG18300.037.0282553
GACCGTT18650.035.5702442
CGACCTA16750.034.7974244
CCGTTGA20500.033.2862934
GTTAGTT69050.027.9981841
TGACGAC22400.027.7112881
TAGGTCG13900.027.2724083
CTTAGCG18700.027.1159258
CGTTGAC26450.026.694235
CTTGACG15950.026.1431
TAACCCC36500.025.5791079
CACGCCG42750.025.2729244
TACACGC42750.025.271812
ACGACGT17150.024.0393417
GCGGGTC36850.023.7887652
GCGAACG9000.023.6929043
TCGTAAG13050.023.23976
TCACCCG41400.023.0061513
CGCGGTC15150.022.5192171
Produced by FastQC (version 0.11.5)