FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10285572
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA179540.1745551924579401No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC121810.11842802714326438No Hit
CTACACGCCGCCGCTTGTGCTGCAGCCATGTCTCTAGTGATCCCTGAAAA108500.10548757035583435No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT13750.041.350821
CGACCTA16400.038.4258884
GACCGTT15950.035.6472552
ACCGTTG16950.035.500923
CTTAGCG17000.031.2200648
CCGTTGA19150.030.6809674
GCGAACG8650.030.1267413
GTTAGTT70650.029.5754951
CACGCCG44550.027.7593294
TACACGC45450.027.1047292
TGACGAC23600.027.1036221
ACGACGT16900.026.6365767
TAACCCC34900.024.170569
GAACGCG11850.022.7914875
TACCCTA90450.022.6867079
ACGCCGC57150.022.6340645
CTACACG56050.022.4859681
GTTTTAC96650.022.159135
GCGGGTC38250.021.9253942
TAGGTCG13200.021.8958323
Produced by FastQC (version 0.11.5)