FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9793291
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC142150.14515038917969456No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC139420.14236276651025687No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG136960.13985084278614818No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA128630.13134501976914606No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT15900.057.805771
GACCGTT19850.045.8257522
ACCGTTG20900.043.9773143
CCGTTGA22400.041.45694
CGTTGAC28800.033.067155
CGACCTA12150.031.9761644
TAACCCC42000.028.544779
TACACGC33450.027.4773272
GTTAGTT59300.025.9654161
CACGCCG35350.025.4655444
GCGAACG7750.024.4529673
CGCAAAA53150.023.9781481
TTAACCC49900.023.9291088
ACGACGT12300.023.8835987
ACCCGGC38050.023.658775
TAGGTCG10900.023.4714833
GCCTATC61750.023.4028856
TCACCCG40200.022.8638293
ACGCCGC42600.022.57775
CCGTATG25600.022.394134
Produced by FastQC (version 0.11.5)