FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9806879
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC142120.14491868411958586No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC138660.14139054840994775No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG134160.13680193260261497No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA130830.13340635690518868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT16500.055.1432841
ACCGTTG19600.046.4216463
GACCGTT20800.044.4269682
CCGTTGA20700.043.9550174
CGTTGAC28150.032.995545
CGACCTA12900.031.2253114
GCGAACG7650.030.3535863
TAGGTCG11650.028.0671633
TAACCCC39500.027.2372939
CACGCCG34150.026.6433054
GCCTATC60950.025.8920146
TACACGC34600.025.7488082
ACCCGGC40050.025.3214665
GTTAGTT61250.024.835581
TCACCCG41800.024.7147123
TTAACCC46750.022.911488
GAACGCG10600.022.800375
ACGACGT12950.022.6890987
CCGTATG24800.022.5479934
ACGCCGC41800.022.4474225
Produced by FastQC (version 0.11.5)