FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827854

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827854
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8511096
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGA104370.12262815505782099No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT85450.10039835057670599No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCGAA25400.038.4627151
TGTCGAG12000.035.227945
CGTACGC28450.030.218635
CCGTACG29050.029.5944944
GGCGAAA12900.028.7199292
GTCGAGG14750.028.6600196
GTACGCC31150.027.9043246
GCGAAAA7750.026.9668033
TACGCCA32950.026.379967
TCCGTAC34100.025.9081883
GTATCGA18900.024.3774574
AGGCGTA41000.024.0967228
CGCGTAT1401.7365184E-523.8281231
TTCCGTA37850.023.5923082
TTCGCGC15750.023.522997
CCCGTAT20250.022.592591
CCCGTCG17150.022.5081981
ACCGCGT4700.022.2332676
TGTGTCG18800.022.2332673
ACGACTT16650.021.7531
Produced by FastQC (version 0.11.5)