FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827856

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827856
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8494297
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGA105670.12440111288785877No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT88770.10450541110111877No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCGAA26150.034.6207161
CGTACGC27750.032.521445
TGTCGAG11700.032.0715835
CCGTACG28250.031.7777024
GGCGAAA12600.031.288642
GTCGAGG12950.031.1765676
GTACGCC29750.030.3351256
TACGCCA32350.028.0438887
GCGAAAA8500.027.3814413
GTATCGA18600.026.8136234
AGGCGTA42800.026.3016958
TCCGTAC34850.026.1684273
CCCGTAT21050.025.1260951
TTCGCGC14900.024.5461627
TATCGAA21650.023.9137545
CCCGTCG16100.023.676541
TTCCGTA39300.023.0844752
CGCGTAA31750.022.8115061
CGCAAGT27050.022.21275536-37
AAGTGCG25350.022.01603138-39
Produced by FastQC (version 0.11.5)