FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827858

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827858
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11120041
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT117210.1054042876280762No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT117010.10522443217610439No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGAG15750.031.0624165
GTATCGA18850.026.7099484
GTCGAGG19150.026.5395496
CCCGTAT24300.023.925661
TATCGAA22000.023.101475
GTGTCGA22850.021.8263764
ACGTACG11550.020.7676852-53
TCGAGGG25650.019.4437717
CGCGTAA11150.017.523441
CGTATCG43750.017.4793953
TGTGTCG28450.017.3631943
ACCCGTT40550.017.3360212
CGTACGA14050.017.24139654-55
CGTACGC30450.017.1586845
CTCCGAT40350.016.6527481
GCTACGT15200.016.56192650-51
CTCACGT51250.016.5514681
CCGTACG31750.016.4561234
TACGTAC15600.015.98502352-53
CCGTATC47450.015.8161012
Produced by FastQC (version 0.11.5)