FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11120041
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCT196830.1770047430580517No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA192580.17318281470365082No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT150280.1351433866116141No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT132800.11942402010927837No Hit
CTACACGCCGCCGCTTGTGCTGCAGCCATGTCTCTAGTGATCCCTGAAAA120760.10859672190057573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT13500.045.6205371
ACCGTTG17850.035.2996253
GACCGTT18250.034.0067442
CGACCTA15350.033.024484
CCGTTGA20650.030.2840844
TACACGC42350.027.9668332
CACGCCG43450.027.5861634
GTTAGTT72100.027.1372381
ACCCGGC43650.027.0256215
CGTTGAC25300.026.0288435
TCACCCG47350.025.013633
ACGACGT16200.024.5664527
CTCGATT29250.024.51855748-49
TAGGTCG14950.024.400923
GCGAACG8550.023.8264313
ACGCCGC55100.023.3011975
TACCCTA84400.022.7374369
TGACGAC23500.022.5788061
CTACACG56000.021.9956171
ATTCCGT32850.021.84178252-53
Produced by FastQC (version 0.11.5)