FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827861

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827861
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11112341
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCT198340.17848624335772273No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA191990.17277187588105875No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT150570.13549800172618895No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT133770.120379675173755No Hit
CTACACGCCGCCGCTTGTGCTGCAGCCATGTCTCTAGTGATCCCTGAAAA121720.10953587547394379No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT13650.044.780471
ACCGTTG17550.035.9093933
GACCGTT17650.035.1688542
CGACCTA13200.034.8206834
CCGTTGA19050.032.087374
TAGGTCG12700.029.4752123
GTTAGTT71900.027.5472981
TACACGC41250.027.3391232
ACCCGGC44200.026.6941745
CACGCCG43000.026.5575524
CGTTGAC24950.025.8290145
TCACCCG47450.025.364783
CTCGATT30250.024.181348-49
CTTGACG16750.023.7627161
CTTAGCG15300.023.5371441
GTCTAAC13500.023.1655061
TACCCTA85300.022.6691199
TGACGAC21050.022.5102081
ACGCCGC54600.022.4774275
GCGAACG10400.022.325253
Produced by FastQC (version 0.11.5)