FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11146991
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCT197190.17689975707345595No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA190460.17086225332020094No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT150100.13465517286234463No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT134560.1207141909417528No Hit
CTACACGCCGCCGCTTGTGCTGCAGCCATGTCTCTAGTGATCCCTGAAAA120490.10809195055418992No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT13300.048.097731
CGACCTA13850.036.266234
ACCGTTG18350.035.3776443
CCGTTGA18150.034.9844064
GACCGTT18600.033.8829422
TAGGTCG11750.031.8591083
GTTAGTT75000.027.546581
CTTAGCG17800.027.4209848
CACGCCG43450.026.7191524
GTCTAAC14250.026.60221
TACACGC42750.026.4913562
CGTTGAC25700.025.813515
ACGACGT15400.024.308747
GCGAACG7650.023.5378363
ACCCGGC41750.023.2674925
TACCCTA86700.023.1760719
TCACCCG44050.022.3749373
CCGTATG20550.022.3669224
TGACGAC23600.022.2870921
ACGCCGC55700.022.1192475
Produced by FastQC (version 0.11.5)