FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9759812
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACGAA418780.42908613403618834No Hit
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACG397430.40721071266536696No Hit
CTCAGGTTCTTGTTTTGCACAGCAGTCAGCCATTTCACCATAGGTTTCAC211150.2163463804425741No Hit
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACAAGCTCAACAAGTGCAGTTT198160.20303669783803213No Hit
CTTCAAATGGACACTGCTGAAGATACTGAGCAAAGGCAATCAACACCAAG171390.17560789080773276No Hit
GGACACTGCTGAAGATACTGAGCAAAGGCAATCAACACCAAGGCTTTGAA168420.17256479940392294No Hit
CTTACTTTCAACAAAATCAGCAGCTAATGAAGGCAAGTCAGCAGGCATCT167690.17181683417672391No Hit
CCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACGA148870.15253367585359226No Hit
CTCCAAAAAGGGTATGAAGTGATTTGTCACAATTTTCAGCTGACTCATCA134950.1382711060417967No Hit
CACTGCTGAAGATACTGAGCAAAGGCAATCAACACCAAGGCTTTGAAATT131500.134736201885856No Hit
CTTTGAAATTTTCTTCTCCCAAATCTTTAAACCGATGAGCAACCTCACTC119530.12247162137959215No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA111760.1145104024544735No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA111170.11390588261331264No Hit
CACAATTTTCAGCTGACTCATCAGCAACACATGTTTTTGCAAATTCAGTT108430.11109845148656552No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT107820.1104734394473992No Hit
CTTCATTGTCATGAAAAGCAGTGCACATCACATCAACCTCTGGTCTCACC98800.10123145814693972No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT98070.10048349291974068No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGAG8700.034.9411855
CCCGTAT18950.030.9426571
GTATCGA18350.030.2849434
GTCGAGG11600.026.205896
CGTACGC20550.026.118225
CCGTACG22050.024.3414694
CTTACTT72400.024.230981
TATCGAA27050.022.827225
TCCGTAC24600.022.3975143
TACGCCA24400.022.1917727
GTACGCC24400.021.9971076
TCGAGGG13500.021.11037
CGTATCG35200.020.7804533
ACGTACG7150.020.59361552-53
CCGTATC37400.020.3200742
CGTACGA7850.019.36231454-55
CGCGTTT2502.0950756E-719.0687271
ATCGAAG51250.018.6285516
TTCCGTA30500.018.3763432
CGAAAGG25350.018.1748458
Produced by FastQC (version 0.11.5)